DeepChek® Assay Whole Genome HIV-1 Genotyping

A whole-genome HIV-1 amplification and NGS workflow intended to broaden resistance-associated mutation coverage across targeted HIV-1 genes.

HIV Genotyping & Drug Resistance

Whole Genome HIV-1 Genotyping

CE-IVDRUO

This assay amplifies the whole genome of HIV-1 from extracted RNA and may support antiviral resistance assessment through detection of resistance-associated mutations in regions such as reverse transcriptase, protease, integrase, and envelope.

Sequencing workflow HIV-1 DeepChek portfolio

DeepChek Assay Whole Genome HIV-1 Genotyping

Key Details

Uses two RT-PCR steps from HIV-1 positive extracted RNA followed by NGS.
Covers representative HIV-1 M group subtypes including A1, A2, B, C, D, F1, F2, G, H, J, K, and L.
Reported LoD is 25,000 cp/mL.
Coverage is approximately 9,600 bp across five amplicons of about 700 to 3,500 bp.
Supports DeepChek software analysis and may be compatible with other HIV-1 tailored downstream NGS software.

Workflow Snapshot

Target regionWhole HIV-1 genome

CoverageApproximately 9,600 bp

Amplicons5 amplicons, about 700 to 3,500 bp

Workflow2 RT-PCR steps followed by NGS

Run formats24, 48, 96, and 384 sample workflows

AnalysisDeepChek Whole Genome HIV-1 software support

Ordering Information

Product	Reference
DeepChek Assay Whole Genome HIV-1 Genotyping (CE-IVD)	170A24
DeepChek Assay Whole Genome HIV-1 Genotyping (RUO)	170A24
DeepChek HIV Software (CE-IVD)	S-12-023 (HL)

Implementation Note

A broader sequencing option for laboratories that want expanded HIV-1 genomic coverage beyond focused PR, RT, or INT panels.

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